Chemical Structure : DDO-4033
CAS No.:
DDO-4033
(DDO4033)
Catalog No.: PC-24933Not For Human Use, Lab Use Only.
DDO-4033 is a potent, selective E3 ligase speckle-type POZ protein (SPOP) inhibitor with IC50 of 16.48 uM, binds to SPOP-MATH with Kd of 15.1 uM in MST assay.
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Purity & Documentation Purity:
>98% (HPLC)
Biological Activity
DDO-4033 is a potent, selective E3 ligase speckle-type POZ protein (SPOP) inhibitor with IC50 of 16.48 uM, binds to SPOP-MATH with Kd of 15.1 uM in MST assay.
DDO-4033 is a potent binder for SPOP with a KD value of 4.96 μM in BLI assay.
DDO-4033 exhibits the corresponding affinity in the ITC experiment with the Kd value of 4.94 μM and no binding to the mutant SPOP-Y87A and D130A.
DDO-4033 (0.5-1 uM) inhibits SPOP-mediated ubiquitination and degradation of LATS1 in ccRCC cells.
DDO-4033 suppresses the malignancy of ccRCC through the Hippo pathway, downregulated the mRNA level of CTGF and CYR61 genes, modulates the downstream YAP/TAZ signaling in ccRCC cells.
DDO-4033 (50 mg/kg) effectively suppresses ccRCC tumor growth in A498-Luc xenograft model.
Physicochemical Properties
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M.Wt
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406.40
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Formula
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C20H18N6O4
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Appearance
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Solid
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CAS No.
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Storage
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- Solide Powder
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-20°C 12 Months; 4°C 6 Months
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- In Solvent
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-80°C 6 Months; -20°C 6 Months
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Shipping
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Solubility
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10 mM in DMSO
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Chemical Name/SMILES
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4-Ethynyl-N-(3-methyl-1-(6-oxo-4-propyl-1,6-dihydropyrimidin-2-yl)-1H-pyrazol-5-yl)-3-nitrobenzamide
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References
1. Zhang Q, et al. J Med Chem. 2025 Jul 7. doi: 10.1021/acs.jmedchem.5c00987.
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