WNA1 is a potent activator of WNT/β-catenin signaling, and conformation-sensitive HDAC1/2 inhibitor, inhibits the HDAC activity of the NuRD complex, while having minimal effect on recombinant HDAC1, inhibits HDAC1-MTA1 complex.
WNA1 increases β-catenin stabilization in preadipocyte 3T3-L1 cells and this activity is blocked by WNT974.
WNA1 suppresses MDI-induced lipid droplet accumulation and the expression of adipocyte differentiation marker Fabp4, which is reversed by cotreatment of WNT974.
WNA1 induces β-catenin stabilization, increases alkaline phosphatase (ALP) activity and ALP expression in ST2 mesenchymal progenitor cells.
WNA1 promotes hair growth, accelerates anagen entry of hair follicles in vivo.
WNA1 inhibits HDAC1/2 complexes, increases β-catenin and acetylated histone H3 levels.
WNA1 can efficiently inhibit HDAC1-MTA1 but only has minor activity on HDAC3-SMRT-DAD in the presence of InsP6.
WNA1 promotes WNT/β-catenin signaling and increases histone acetylation through binding to the foot pocket of HDAC1/2.
WNA1 functions primarily by promoting endogenous WNT expression or signaling.