Catalog No.: PC-26142Not For Human Use, Lab Use Only.
CCKBR agonist MF-8 is a potent, highly β-arrestin biased CCKBR (Cholecystokinin B Receptor, CCK2) agonist with binding IC50 of 0.9 nM and EC50 of 516 nM in calcium signaling assays, performs poorly in activating Gαq/11-Ca2+ signaling but is highly potent in inducing β-arrestin2 signaling.
CCKBR agonist MF-8 is a potent, highly β-arrestin biased CCKBR (Cholecystokinin B Receptor, CCK2) agonist with binding IC50 of 0.9 nM and EC50 of 516 nM in calcium signaling assays, performs poorly in activating Gαq/11-Ca2+ signaling but is highly potent in inducing β-arrestin2 signaling.
MF-8 binds to CCKBR with high affinity (IC50=0.9 nM), shows IC50 of 4.54 nM in PRESTO-Tango assays.
MF-8 does not exhibit any agonistic effect on the Gαq/11 signaling of CCKAR and β-arrestin1/2 downstream of GPR173.
MF-8 can effectively induce endocytosis of CCKBR. MF-8 inhibited the key signaling pathway of CCKBR-mediated calcium, cAMP accumulation, and LTP.
MF-8 inhibits the increase in calcium flux and cAMP accumulation induced by CCK8s with IC50 of 22.7 nM and 16 nM in 293T-CCKBR cells.
MF-8 inhibits the CCKBR-LTP mediated cue-to-cue fear memory.
