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Evans Blue

Chemical Structure : Evans Blue

CAS No.: 314-13-6

Evans Blue (Direct Blue 53, T-1824, C.I. 23860)

Catalog No.: PC-24513Not For Human Use, Lab Use Only.

Evans Blue is a selective small-molecule inhibitor of CaMKII-alpha (CaMKII-α) with IC50 of 197.1 nM, also is a potent inhibitor of L-glutamate uptake via the membrane bound excitatory amino acid transporter (EAAT).

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Purity & Documentation Purity: >98% (HPLC) Select Batch:

Biological Activity

Evans Blue is a selective small-molecule inhibitor of CaMKII-alpha (CaMKII-α) with IC50 of 197.1 nM, also is a potent inhibitor of L-glutamate uptake via the membrane bound excitatory amino acid transporter (EAAT).
Evans Blue is a L-glutamate and kainate receptor-mediated currents inhibitor.
Evans Blue has a strong affinity towards serum albumin, as a high molecular weight protein tracer.
Evans Blue is also used to study BBB (blood-brain barrier) permeability.

Physicochemical Properties

M.Wt 960.79
Formula C34H24N6Na4O14S4
Appearance Solid
CAS No.
Storage
Solide Powder
-20°C 12 Months; 4°C 6 Months
In Solvent
-80°C 6 Months; -20°C 6 Months
Shipping
Solubility

10 mM in DMSO

Chemical Name/SMILES

Sodium 6,6'-((3,3'-dimethyl-[1,1'-biphenyl]-4,4'-diyl)bis(diazene-2,1-diyl))bis(4-amino-5-hydroxynaphthalene-1,3-disulfonate)

References

1. Tian J, et al. Cancer Sci. 2025 Apr 4. doi: 10.1111/cas.70051.

2. Linpeng Yao, et al. Contrast Media Mol Imaging. 2018 Apr 22:2018:7628037.

3. Anatol Manaenko, et al. J Neurosci Methods. 2011 Feb 15;195(2):206-10.

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