KN-93 is a selective, cell-permeable, reversible and competitive inhibitor calmodulin-dependent kinase type II (CaMKII) with Ki of 0.37 uM. KN-93 shows no significant effects on the catalytic activity of cAMP-dependent protein kinase, Ca2+/phospholipid dependent protein kinase, myosin light chain kinase and Ca(2+)-phosphodiesterase. KN-93 also inhibits the autophosphorylation of both the alpha- and beta-subunits of CaMKII。 KN-93 decreases dopamine (DA) levels in PC12h cells, significantly suppressed KCl or acetylcholine-induced tyrosine hydroxylase (TH) phosphorylation.
