ML-SI3 is a synthetic inhibitor of TRPML calcium channel blocker with IC50 of 4.7 μM and 1.7 μM for TRPML1/2 channel respectively.
ML-SI3 blocks CCCP- and H2O2-induced autophagosome formation, suppresses CCCP-induced LC3-PARKIN co-localization (mitophagy).
(-)-isomer ML-SI3 is a potent inhibitor of TRPML1 and TRPML2 (IC50 values 1.6 and 2.3 μM) and a weak inhibitor (IC50 12.5 μM) of TRPML3.
The (+)-enantiomer ML-SI3 is an inhibitor on TRPML1 (IC50 5.9 μM), but an activator on TRPML 2 and 3.
