USP28 inhibitor 19 is a potent, selective USP28 inhibitor with IC50 of 1.1 uM, does not inhibit USP7 and LSD1 (IC50>100 uM).
USP28 inhibitor 19 exerted good cell inhibitory activities against HGC-27 and MKN45 cells (IC50=0.61 and 1.49 μmol/L, respectively).
USP28 inhibitor 19 (20 uM) decreased expression of USP28 in HGC-27 cells, thus leading to decreased expression of its substrates LSD1 and total c-Myc.
USP28 inhibitor 19 induced cellular degradation of USP28, LSD1 and total c-Myc in HGC-27 cells.
USP28 inhibitor 19 inhibited colony formation and proliferation of gastric cancer cells, induced cell apoptosis and cell cycle arrest in HGC-27 cells.
