USP7 inhibitor ALM4 (AD-04) is a highly potent, selective, allosteric USP7 inhibitor with IC50 of 6 nM in FP assays, SPR Kd of 2 nM.
AD-04 also shows high potency in an alternative assay using the Ub-Rh110 substrate (IC50=1.5 nM).
AD-04 displays high selectivuty (>38-fold) against other DUBs, proteases and kinases.
AD-04 demonstrates highly potent target engagement in cells (EC50=49 nM), with no off-target activity against USP47, causes nongenotoxic stabilization of p53 and decreased levels of MDM2 in HCT116 cells.
